CK100402S Recombinant Human IL-21 (CHO)

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Animal-Free Recombinant Proteins for CGT Manufacturing

50-Ton Annual rHSA Capacity. Animal-Free. Industrial Scale.

Animal-Free Recombinant Proteins for CGT Manufacturing

50-Ton Annual rHSA Capacity. Animal-Free. Industrial Scale.

Animal-Free Recombinant Proteins for CGT Manufacturing

50-Ton Annual rHSA Capacity. Animal-Free. Industrial Scale.

Animal-Free Recombinant Proteins for CGT Manufacturing

50-Ton Annual rHSA Capacity. Animal-Free. Industrial Scale.

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CK100402S Recombinant Human IL-21 (CHO)

CK100402S Recombinant Human IL-21 (CHO cell-expressed) from SciProTech is manufactured using a CHO cell animal-free expression system, delivering high activity, high purity, and excellent batch-to-batch consistency. IL-21 is an important cytokine regulating NK cell and T cell proliferation, differentiation, and functional maintenance, and is widely used in NK cell expansion, CAR-NK manufacturing, T cell culture, CAR-T process development, and tumor immunology research. When used in combination with cytokines such as IL-2, IL-7, and IL-15, it can further enhance immune cell expansion efficiency and functional activity, meeting the demands of cell and gene therapy (CGT) R&D and industrial-scale production for high-quality cytokines.
Lyophilized powder form, convenient for transport, storage, and culture system preparation. Suitable for research, process development, and biomanufacturing applications.
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Product parameters

Product Introduction

CK100402S Recombinant Human IL-21 (CHO cell-expressed) from SciProTech is manufactured using a CHO cell animal-free expression system, delivering high activity, high purity, and excellent batch-to-batch consistency. IL-21 is an important cytokine regulating NK cell and T cell proliferation, differentiation, and functional maintenance, and is widely used in NK cell expansion, CAR-NK manufacturing, T cell culture, CAR-T process development, and tumor immunology research. When used in combination with cytokines such as IL-2, IL-7, and IL-15, it can further enhance immune cell expansion efficiency and functional activity, meeting the demands of cell and gene therapy (CGT) R&D and industrial-scale production for high-quality cytokines.

Lyophilized powder form, convenient for transport, storage, and culture system preparation. Suitable for research, process development, and biomanufacturing applications.

Background

Interleukins are a class of cytokines produced by and acting on multiple cell types. They play important roles in signal transduction, activation and regulation of immune cells, mediation of T and B cell activation, proliferation and differentiation, and inflammatory responses. IL-21 (interleukin-21) is secreted by activated CD4⁺ T cells, with a molecular structure similar to IL-15. It participates in regulating B cell proliferation and synergizes with IL-15 to promote bone marrow precursor cell proliferation and NK cell proliferation, differentiation, and cytotoxic activity. This product is expressed in CHO cells, featuring high purity, high activity, and freedom from animal-derived contamination.

Product Advantages

CHO cell expression
High-activity recombinant human IL-21
Animal-free production system
High purity, low endotoxin
Excellent batch-to-batch consistency
Suitable for CGT process development
Supports research and industrial-scale production

Product Specification

Parameter	Specification
Product Name	Recombinant Human IL-21
Synonyms	IL21, IL-21, Interleukin 21, Interleukin-21, Za11
Product Number	CK100402S
Specification	50 μg / 1 mg
Product Information
Accession Number	Q9HBE4
Gene ID	59067
Amino Acid Sequence	QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLK RKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS
Expression Host	CHO
Molecular Weight	15 kDa
Tag	C-His Tag
Form and Formulation	Sterile-filtered through a 0.2 μm filter, lyophilized from PBS (NaCl: 137 mM, KCl: 2.7 mM, Na₂HPO₄: 10 mM, KH₂PO₄: 1.8 mM)
Purification Method	Chromatography
Storage Condition	-20°C to -80°C
Product Quality
Sterility Test	Pass
Endotoxin	≤ 10 EU/mg
Purity	≥ 98%, SDS-PAGE (Figure 1)
Biological Activity	NK-92 cell IFN-γ secretion assay, ED50 ≤ 0.5 ng/mL, corresponding specific activity ≥ 2×10⁶ U/mg (Figure 2)
Host Cell Protein Residue	< 100 ng/mg
Mycoplasma Test	Pass

IL-21 SDS-PAGE

Figure 1： Recombinant Human IL-21 purity test results, SDS-PAGE (sample NR, non-reduced, sample R, reduced)

IL-21 Bioactivity - NK-92 IFN-γ secretion assay

Figure 2： Recombinant Human IL-21 biological activity test results, activity >2×10⁶ IU/mg

Directions for Use

This product is in lyophilized powder form. It is recommended to reconstitute with sterile water, PBS, or other suitable buffer to prepare a stock solution at 0.1–1.0 mg/mL.

After complete dissolution, further dilute to working concentration using culture medium according to experimental requirements. Aliquot for single-use storage and store at -20°C or below. Avoid repeated freeze-thaw cycles to maintain product activity and stability.

Optimal cytokine concentration may vary across different cell types and culture systems. Optimization and validation based on specific culture processes and experimental objectives are recommended to achieve the best culture results.

Transport & Storage

Ice pack transport.

Store at -20°C or below, protected from light, moisture-proof, and sealed. After reconstitution, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles. Minimize product exposure to room temperature during handling to ensure product activity and stability.

Applications

Recombinant Human IL-21 is an important member of the γc family of cytokines, regulating immune cell proliferation, differentiation, and functional activation through IL-21R-mediated JAK/STAT signaling pathways.

Widely used in:

NK cell expansion and functional enhancement
T cell culture and activation
CAR-T cell manufacturing process development
CAR-NK cell culture
Tumor immunology research
Immune cell therapy process development
Cytokine functional research
Biopharmaceutical R&D

IL-21 is often used in combination with cytokines such as IL-2, IL-7, and IL-15 to promote NK cell and CD8⁺ T cell expansion and to help maintain cytotoxic activity and memory phenotype.

References

1. Rosanne Spolski and Warren J. Leonard. Interleukin-21: Basic Biology and Implications for Cancer and Autoimmunity. Annual Review of Immunology 2008 26:1, 57-79.

2. Spolski, R., Leonard, W. Interleukin-21: a double-edged sword with therapeutic potential. Nat Rev Drug Discov 13, 379-395 (2014).